A LOW-COST AND EASY TO USE SORTING DEVICE FOR THE SEPARATION OF EXTRACELLULAR VESICLES FROM COMPLEX FLUIDS

International audienceIdentifying and characterizing Extracellular vesicles (EVs) in biofluids could lead to unprecedented diagnosis/prognosis assays for a wide set of pathologies. Owe to their complexity in size, origin, membrane markers, there is currently no ideal technology available yet to sort, to quantify and characterize cell-derived microvesicles, and fully relate their structure and functions which have high potential in clinical diagnostics and early treatment of life threatening diseases such as cancer, cardio vascular diseases…. Lab-on-chip for EVs size-based separation Chip EVs capture Sample EVs characterization (AFM imaging, mass spectrometry) APPROACH We propose to couple a microfluidic device (LOC) enabling to separate particles from a complex sample to a device allowing the capture of particles on specific spots and their analysis. These methods allow obtaining important information on a chip about nanoscale vesicles in terms of size, membrane markers expression, and structure-function properties