To date, exon capture has largely been restricted to species with fully sequenced genomes, which has precluded its application to lineages that lack high quality genomic resources. We developed a novel strategy for designing array-based exon capture in chipmunks (Tamias) based on de novo transcriptome assemblies. We evaluated the performance of our approach across specimens from four chipmunk species.ResultsWe selectively targeted 11,975 exons (~4 Mb) on custom capture arrays, and enriched over 99% of the targets in all libraries. The percentage of aligned reads was highly consistent (24.4-29.1%) across all specimens, including in multiplexing up to 20 barcoded individuals on a single array. Base coverage among specimens and within targets ...
Evidence from natural systems suggests that hybridization between animal species is more common than...
Natural history museum collections provide unique resources for understanding how species respond to...
Exon capture across species has been one of the most broadly applied approaches to acquire multi-loc...
To date, exon capture has largely been restricted to species with fully sequenced genomes, which has...
AbstractBackgroundTo date, exon capture has largely been restricted to species with fully sequenced ...
Background: To date, exon capture has largely been restricted to species with fully sequenced genome...
BACKGROUND To date, exon capture has largely been restricted to species with fully sequenced genomes...
Exon capture coupled to high-throughput sequencing constitutes a cost-effective technical solution f...
Exon capture coupled to high-throughput sequencing constitutes a cost-effective technical solution f...
Targeted exons were derived from 6,249 protein-coding genes with a target size totaling 4Mb, and spa...
The evolutionary histories of species are not measured directly, but estimated using genealogies inf...
The evolutionary histories of species are not measured directly, but estimated using genealogies inf...
The evolutionary histories of species are not measured directly, but estimated using genealogies inf...
Custom sequence capture experiments are becoming an efficient approach for gathering large sets of o...
Custom sequence capture experiments are becoming an efficient approach for gathering large sets of o...
Evidence from natural systems suggests that hybridization between animal species is more common than...
Natural history museum collections provide unique resources for understanding how species respond to...
Exon capture across species has been one of the most broadly applied approaches to acquire multi-loc...
To date, exon capture has largely been restricted to species with fully sequenced genomes, which has...
AbstractBackgroundTo date, exon capture has largely been restricted to species with fully sequenced ...
Background: To date, exon capture has largely been restricted to species with fully sequenced genome...
BACKGROUND To date, exon capture has largely been restricted to species with fully sequenced genomes...
Exon capture coupled to high-throughput sequencing constitutes a cost-effective technical solution f...
Exon capture coupled to high-throughput sequencing constitutes a cost-effective technical solution f...
Targeted exons were derived from 6,249 protein-coding genes with a target size totaling 4Mb, and spa...
The evolutionary histories of species are not measured directly, but estimated using genealogies inf...
The evolutionary histories of species are not measured directly, but estimated using genealogies inf...
The evolutionary histories of species are not measured directly, but estimated using genealogies inf...
Custom sequence capture experiments are becoming an efficient approach for gathering large sets of o...
Custom sequence capture experiments are becoming an efficient approach for gathering large sets of o...
Evidence from natural systems suggests that hybridization between animal species is more common than...
Natural history museum collections provide unique resources for understanding how species respond to...
Exon capture across species has been one of the most broadly applied approaches to acquire multi-loc...