This dissertation focuses on the folding dynamics of a bacterial membrane protein, Outer Membrane Protein A (OmpA), using fluorescence and Ultraviolet resonance Raman spectroscopy. Our model [Beta]-barrel membrane protein, OmpA, contains five native anchoring Tryptophan residues. The spectroscopic properties of trp residues are highly sensitive to the local environment, making it an ideal probe for membrane protein folding studies. Utilizing trp fluorescence, refolding studies were performed on single trp mutants of OmpA to determine the thermodynamic stability of these trp mutants. The important noncovalent interactions that promote stability in OmpA are pairwise aromatic interactions and hydrogen bonds with the N₁H moiety of trp. Refoldin...
AbstractThe folding reaction of a β-barrel membrane protein, outer membrane protein A (OmpA), is pro...
ABSTRACT: The mechanism of insertion and folding of an integral membrane protein has been investigat...
Spectroscopic investigations of protein folding mechanism(s) require the elucidation of the relation...
This dissertation focuses on the folding dynamics of a bacterial membrane protein, Outer Membrane Pr...
A 325 residue integral membrane protein, outer membrane protein A (OmpA), has been investigated usin...
Approximately 30 % of human genes encode for membrane proteins. Membrane proteins play important rol...
AbstractAromatic amino acids of membrane proteins are enriched at the lipid-water interface. The rol...
Ultraviolet resonance Raman (UVRR) spectroscopy is a vibrational technique that reveals structures a...
Membrane proteins constitute a significant fraction of cellular proteins due to their vast diversity...
Membrane proteins constitute a significant fraction of cellular proteins due to their vast diversity...
AbstractAromatic amino acids of membrane proteins are enriched at the lipid-water interface. The rol...
This project investigates changes in protein solvation during the folding reaction of Outer membrane...
An understanding of protein folding and how it impacts the structure and function of proteins will h...
An understanding of protein folding and how it impacts the structure and function of proteins will h...
This project investigates changes in protein solvation during the folding reaction of Outer membrane...
AbstractThe folding reaction of a β-barrel membrane protein, outer membrane protein A (OmpA), is pro...
ABSTRACT: The mechanism of insertion and folding of an integral membrane protein has been investigat...
Spectroscopic investigations of protein folding mechanism(s) require the elucidation of the relation...
This dissertation focuses on the folding dynamics of a bacterial membrane protein, Outer Membrane Pr...
A 325 residue integral membrane protein, outer membrane protein A (OmpA), has been investigated usin...
Approximately 30 % of human genes encode for membrane proteins. Membrane proteins play important rol...
AbstractAromatic amino acids of membrane proteins are enriched at the lipid-water interface. The rol...
Ultraviolet resonance Raman (UVRR) spectroscopy is a vibrational technique that reveals structures a...
Membrane proteins constitute a significant fraction of cellular proteins due to their vast diversity...
Membrane proteins constitute a significant fraction of cellular proteins due to their vast diversity...
AbstractAromatic amino acids of membrane proteins are enriched at the lipid-water interface. The rol...
This project investigates changes in protein solvation during the folding reaction of Outer membrane...
An understanding of protein folding and how it impacts the structure and function of proteins will h...
An understanding of protein folding and how it impacts the structure and function of proteins will h...
This project investigates changes in protein solvation during the folding reaction of Outer membrane...
AbstractThe folding reaction of a β-barrel membrane protein, outer membrane protein A (OmpA), is pro...
ABSTRACT: The mechanism of insertion and folding of an integral membrane protein has been investigat...
Spectroscopic investigations of protein folding mechanism(s) require the elucidation of the relation...