Comparative HPLC analysis of different samples of Tribhuvankirti Rasa

Background: There are different methods of Vatsanabha shodhana but it is not mentioned in literature that which method should be used in preparation of Tribhuvankirti rasa which will make the drug safer. Objectives: To acquire knowledge regarding toxic principle in self prepared and market sample of Tribhuvankirti Rasa with respect to different shodhana methods of Vatsanabha and to develop a method of testing toxic principle in the formulation. Material and methods: Three samples were prepared out of which two samples were prepared using different shodhana media and one sample was prepared using impure ingredients and three samples of Tribhuvankirti Rasa were purchased from market. Physicochemical and phytochemical analysis of the final product was conducted and the observations were compared among six samples. Results: HPLC graphs indicate that the numbers of peaks are increased in the sample prepared with Godugdda shodhita Vatsanabha and all the market samples in comparison with Ashodhita and Gomutra shodhita samples of Tribhuvankirti Rasa. All samples contain alkaloids, however after purification there was decrease in the concentration of detected alkaloid. Conclusion: The toxic principle (Aconitine) in market and self prepared sample of Tribhuvankirti Rasa has been reduced by shodhana. In addition, flavonoids, glycosides and anti-oxidants are found which are beneficial for health