Molecular analysis of human papillomaviruses and HPV 16 variants in patients with high-grade cervical intraepithelial lesion and cervical carcinoma

Cilj istraživanja bio je analizirati distribuciju HR-HPV tipova u bolesnica s HSIL i karcinomom vrata maternice, te utvrditi učestalost HPV 16 genomskih inačica u premalignim i malignim promjenama. HPV-DNA je izolirana iz 373 uzorka pacijentica s HSIL i karcinomom vrata maternice. Najčešći tip u HSIL bio je HPV 16 (57,9%), zatim HPV 31, HPV 33, HPV 58, HPV 52, HPV 18 i HPV 45 (12,8%, 6,8%, 6,4%, 5,9%,4,6% i 3,2%). HPV 16 je bio dominantan u karcinomu pločastih stanica (66,7%), ostali tipovi su bili: HPV 31, HPV 45, HPV 18 i HPV 33 (14,5%, 7,2%, 7,2% i 5,8%) uzorka. U adenokarcinomu HPV 16 bio je u 50,0%, HPV 18 u 41,7% a HPV 45 u 5,6% uzoraka. Određene su HPV16 inačice za 130 HPV16 pozitivnih uzoraka. U 74 uzorka HSIL-a pronađena je 41 inačica, 98,6% pripadalo je europskoj grani, a jedna (1,4%) afričkoj grani. Promatrajući uzorke karcinoma, 95% izolata svrstano u 41 varijantu pripadalo je europskoj grani, jedan izolat (2,5%) u Sjeverno Američkoj i jedan (2,5%) azijsko-američkoj grani. HPV 16 je bio dominantan. HPV 16 varijante dominantno pripadaju europskoj grani bez obzira na patohistološku dijagnozu. Sveobuhvatnim cijepljenjem devetvalentnim HPV cjepivom mogla bi se prevenirati 91,5% premalignih i malignih llezija vrata maternice u Hrvatskoj.The aim of the study was to analyze the distribution of HR-HPV types in patients with HSIL and cervical carcinoma and to determine the incidence of HPV 16 genomic variants in premalignant and malignant changes. HPV-DNA was isolated from 373 samples from patients with HSIL and cervical carcinoma. The most common type in HSIL was HPV 16 (57.9%), followed by HPV 31, HPV 33, HPV 58, HPV 52, HPV 18 and HPV 45 (12.8%, 6.8%, 6.4%, 5.9%, 4.6% and 3.2%). HPV 16 was dominant in squamous cell carcinoma (66.7%), other types were: HPV 31, HPV 45, HPV 18 and HPV 33 (14,5%, 7,2%, 7,2% and 5,8%). In adenocarcinoma HPV 16 was in 50.0%, HPV 18 in 41.7% and HPV 45 in 5.6% samples. HPV16 variants were determined for 130 HPV16 positive samples. In 74 HSIL samples 41 variants were found, 98.6% belonged to the European branch, one (1.4%) to the African branch. Observing carcinoma samples, 95% isolates grouped in 41 variants belonged the European branch, one isolate (2.5%) the North American, and one (2.5 %) the Asian-American branch. HPV 16 was dominant. HPV 16 variants dominantly belong to European branch regardless of pathohistological diagnosis. Comprehensive vaccination with the nine-valent HPV vaccine could prevent 91.5% of premalignant and malignant cervical lesions in Croatia