Evaluasi Suplementasi Indigofera zollingeriana Sebagai Sumber Green Protein concentrate Terhadap Produksi Gas Metan, Amonia dan Sintesis Protein Mikroba Rumen

The use of protein with low-cost, high quality, low methane, and ammonia emissions are a prerequisite as a protein source in ruminant. However, the European Commission has prohibited protein derived from fish meals for ruminant feeds. So encouraging efforts to explore the other protein sources to be most important. Most of the high protein legumes grow in tropical areas such as Indonesia and have the potential as an alternative protein source in ruminant feed, including Indigofera zollingeriana (25-27% protein content). But many browse legumes with high protein are a heterogeneous group of plants, with variable secondary metabolic content and rumen degradable protein. The aim of this experiment was to evaluate the characteristics fermentation of IZ as green protein supplement on in vitro methane, ammonia and microbial protein production. The experiment was a completely randomized design with four different level supplementation of Indigofera zollengeriana (IZ) as green protein concentrate and five replications. The treatment diets were R0; basal diet (60% forage + 40% concentrate) + 0% IZ, R1; R0 + 10% IZ, R2; R0 + 20% IZ, and R3; R0 + 30% IZ. The experiment result showed that supplemenatation of IZ was significant effects (P<0.05) to increase total gas, ammonia (N-NH3), total volatile fatty acid (TVFA), and metabolizable energy (ME) and significant effect (P