Aligned carbon nanotube films for immobilization of glucose oxidase and its application in glucose biosensor

Glucose oxidase (GOx) was immobilized between aligned carbon nanotube films (ACNTFs) using a cross-stackingmethod without any purification and functionalization processes. Direct electron transfer of GOx was achieved on theACNTFs with reversible redox peaks observed at the formal potential of –0.448 V. When applied to the determination ofglucose by cyclic voltammetry, the prepared enzymatic electrode exhibited a linear range from 1 to 5 mM under mediator-free physiological conditions, which makes it a promising candidate for the development of third-generation glucosebiosensors