Additional file 1 of N6-methyladenosine-modified circIGF2BP3 inhibits CD8+ T-cell responses to facilitate tumor immune evasion by promoting the deubiquitination of PD-L1 in non-small cell lung cancer

Additional file 1: Figure S1. Identification of the circRNA module negatively associated with CD8+ TIL infiltration by WGCNA. A. Analysis of network topology for soft powers to identify the threshold best fit in the scale-free network. A soft power of 20 was selected to meet the threshold of 0.85. B. Heatmap summarizing the proportions of infiltrated immune cell subpopulations in five NSCLC samples in GSE126533 analyzed by CIBERSORT. C. Heatmap illustrating the module associations. Red denotes a significant correlation between corresponding modules. D. Scatter plot showing the relationship between gene significance and module membership of circRNAs in the turquoise module. circRNAs enclosed in the red box (gene significance > − 0.96, module membership value > 0.96 and q weighted < 0.01) were selected as hub circRNAs. E. Relative expression levels of circIGF2BP3 in a panel of NSCLC cell lines, a human bronchial epithelial cell line (16HBE), a human lung epithelial cell line (BEAS-2B) and immune cells (CD45+, CD3+, CD4+ and CD8+) isolated from PBMCs. Data represent the mean ± SD