Additional file 1: of CircPSMC3 suppresses the proliferation and metastasis of gastric cancer by acting as a competitive endogenous RNA through sponging miR-296-5p

Figure S1. (a) A total of 6 circRNAs based on the multiple fold difference in circRNA microarray and then were verified in a small sample of plasmas by using qRT-PCR. (b) SiRNA against circPSMC3 were designed to silence circPSMC3 level in BGC823 cells. (c) SiRNA against circPSMC3 were designed to silence circPSMC3 level in SGC7901 cells. (d) SiRNA#1 against circPSMC3 were designed to silence circPSMC3 without influencing PSMC3 mRNA level in BGC823 and SGC7901cells. (e) The expression of circPSMC3 was evaluated in MGC803 and AGS cells transfected with circPSMC3 vector or Mock by using qRT-PCR. (f) The expression of PSMC3 mRNA was evaluated in MGC803 and AGS cells transfected with circPSMC3 vector or Mock by using qRT-PCR. (g) The expression of miR-296-5p was evaluated in MGC803 and AGS cells transfected with miR-296-5p inhibitor or miR-NC. (h) The expression of miR-296-5p was evaluated in MGC803 and AGS cells transfected with with miR-296-5p mimics or miR-NC. *p < 0.05, **p < 0.01, ***p < 0.001. (DOCX 722 kb