Comparison of the domain of Orco accumulation with that of cytoskeleton, membrane and kinesin-2 motor.

(A) Ciliary localisation of Endo-Orco:GFP, cytoskeleton markers- GFP:Tubulin84B and Jupiter:GFP; soluble protein- eGFP; membrane marker- mCD8:GFP; and ciliary trafficking machinery- Klp64D:GFP and Oseg2:GFP, at 6 hours AE. Except for Jupiter:GFP (protein trap) and Endo-Orco:GFP (fosmid line), all the oher transgene expressions were driven by the chaGal4. (B-D) Plot profiles of the distribution (mean ± SEM) of Endo-Orco:GFP and the other ciliary markers along the ciliary OS inside ab1-type s. basiconica at 6 hours AE. The pairwise significance of difference was estimated at every 1 μm interval along the length of the cilia using one-way ANOVA test, p-values (*p (TIF)