Kinesin-2 is necessary for Orco entry into the olfactory cilia.

(A-D) Total fluorescence intensity (mean ± S.E.M) of Endo-Orco:GFP in the ciliary OS and the dendritic knob region of OSNs in Control (A), Klp64D RNAi (B), Klp68D RNAi (C) and homozygous Klp64Dkj353 mutant (D) backgrounds, respectively. The pairwise significance of difference as compared to the wild type control and between different stages was estimated using one-way ANOVA test, and the p-values (*p s. basiconica from Control (orcobGal4, Endo-Orco:GFP/+; UAS-Dicer/+), Klp64D RNAi (orcoGal4, Endo-Orco:GFP/UAS-Klp64D RNAi; UAS-Dicer/+), Klp68D RNAi (orcoGal4, Endo-Orco:GFP/UAS-Klp68D RNAi; UAS-Dicer/+) and homozygous Klp64Dkj353 mutant (Endo-Orco:GFP; Klp64Dkj353) backgrounds at 12 hours AE (a). The histograms depict total fluorescence intensity (mean ± S.D.) of Endo-Orco:GFP in control, the kinesin-2 knock downs, and homozygous Klp64Dkj353 mutant backgrounds at 12 hours AE (b). The pairwise significance of difference was estimated using the one-way ANOVA test, and p-values (*p Klp64Dkj353 mutant backgrounds (also see S6 Fig). The cilia were photobleached during 4–5 hours AE. Examples of prebelach and postbleach images are shown in panel a, and the corresponding quantification is presented in panel b. The pairwise significance of difference as compared to the wild type control was estimated using Mann-Whitney U-test, and the p-values (*p < 0.05, **p < 0.01, and ***p<0.001) are indicated on the box plots. All images are shown in the false colour intensity heat map (FIRE, ImageJ). Scale for images—2 μm (A-D), 10 μm (E-a), and 2 μm (F-a). Number of sensillae (n) quantified is indicated on the graphs.