The PilZ_Δ107-117-FimX_GGDEF-EAL-c-di-GMP complex.

A and B) Ribbon representations of the two main modes of contact observed in the crystal lattice of the PilZΔ107-117-FimXGGDEF-EAL-c-di-GMP complex. PilZΔ107–117 is colored blue and FimXGGDEF-EAL colored magenta. A: PilZ-FimX interface 1. B: PilZ-FimX interface 2. No density for the FimX GGDEF domain was observed and is therefore missing from the model. C) 2FO-FC electron density map (contoured at 1.0 σ) for the PilZΔ107-117-FimXGGDEF-EAL-c-di-GMP structure in the region around the c-di-GMP ligand (shown in stick). D) 2FO-FC electron density map (contoured at 1.0 σ) for inter subunit contacts at interface 1 in the PilZΔ107-117-FimXGGDEF-EAL-c-di-GMP structure in the region around the c-di-GMP ligand (shown in stick). E) Depiction of the crystal lattice along the a and b (left), a and c (center) and b and c (right) axes. Here, the coloring scheme is the same as in A except that the first helix from the EAL domain (residues 436–454) are colored black. (TIF)