The amount of HK remains the same in all samples, while the amount of GK present in the parental sample is diluted with the GK-depleted RNAiGK.i sample. The HK and GK activity were determined in the presence of both glucose and glycerol, as performed in the Glc/Glyc conditions (see Fig 2B). (DOCX)</p
<p>Zymogram analysis of GS activity from wild-type and mutant cells. Ten µg of each soluble extract ...
<p>Comparison of metabolic fluxes in parental, induced low-producing and induced high-producing cell...
<p>NG(normal glucose), HG(high glucose), HM(5.5 mmol/L glucose plus 24.5 mmol/L mannitol), HBCM7(hig...
Enzymatic assays used for the quantification of hexokinase (HK) and glycerol kinase (GK) activity. T...
Cells were maintained in the exponential growth phase (between 106 and 107 cells/mL), and cumulative...
Panel A shows growth curves of the parental 427 90–13 BSF (WT) and RNAiGK cell lines incubated in CM...
a.i: RNAi cell line induced during 5 days by addition of tetracycline; .ni: non-induced RNAi cell li...
<p>(<b>A</b>) Representative images of the color of the growth media 48 h after seeding with 3 × 10<...
(A) Glycerol kinase (GK), hexokinase (HK), and malic enzyme (ME) activity determined in total cell e...
<p>(A-C) Naïve CD4 T cells from WT and HK2 KO mice were activated for 72 hours. (A) mROS (MitoSOX hi...
<p>(A) ARPE-19 cells were transduced with Ad vectors expressing gB, gB and gH/gL, or gH/gL and GFP f...
<p>Glucose consumption (A, D) and lactate formation (B, E) were measured 2 days after transfection w...
<p>Plotted are growth rates (open symbols, right Y axis) and levels of PGK enzymatic activity in cru...
(a) In immunofluorescence microscopy, IHOK-P cells expressed lower level of Vimentin and higher leve...
The extracellular PBS medium of trypanosomes incubated in the presence of 4 mM concentrations of 1 o...
<p>Zymogram analysis of GS activity from wild-type and mutant cells. Ten µg of each soluble extract ...
<p>Comparison of metabolic fluxes in parental, induced low-producing and induced high-producing cell...
<p>NG(normal glucose), HG(high glucose), HM(5.5 mmol/L glucose plus 24.5 mmol/L mannitol), HBCM7(hig...
Enzymatic assays used for the quantification of hexokinase (HK) and glycerol kinase (GK) activity. T...
Cells were maintained in the exponential growth phase (between 106 and 107 cells/mL), and cumulative...
Panel A shows growth curves of the parental 427 90–13 BSF (WT) and RNAiGK cell lines incubated in CM...
a.i: RNAi cell line induced during 5 days by addition of tetracycline; .ni: non-induced RNAi cell li...
<p>(<b>A</b>) Representative images of the color of the growth media 48 h after seeding with 3 × 10<...
(A) Glycerol kinase (GK), hexokinase (HK), and malic enzyme (ME) activity determined in total cell e...
<p>(A-C) Naïve CD4 T cells from WT and HK2 KO mice were activated for 72 hours. (A) mROS (MitoSOX hi...
<p>(A) ARPE-19 cells were transduced with Ad vectors expressing gB, gB and gH/gL, or gH/gL and GFP f...
<p>Glucose consumption (A, D) and lactate formation (B, E) were measured 2 days after transfection w...
<p>Plotted are growth rates (open symbols, right Y axis) and levels of PGK enzymatic activity in cru...
(a) In immunofluorescence microscopy, IHOK-P cells expressed lower level of Vimentin and higher leve...
The extracellular PBS medium of trypanosomes incubated in the presence of 4 mM concentrations of 1 o...
<p>Zymogram analysis of GS activity from wild-type and mutant cells. Ten µg of each soluble extract ...
<p>Comparison of metabolic fluxes in parental, induced low-producing and induced high-producing cell...
<p>NG(normal glucose), HG(high glucose), HM(5.5 mmol/L glucose plus 24.5 mmol/L mannitol), HBCM7(hig...