Role of glycerol kinase in glycerol metabolism.

Panel A shows growth curves of the parental 427 90–13 BSF (WT) and RNAiGK cell lines incubated in CMM_Glc (Glc) or CMM_Glyc/GlcNAc (Glyc), in the presence (.i) or not (.ni) of tetracycline. The western blot controls with the anti-GK and anti-hsp60 (heat shock protein 60) immune sera are shown in the panel B and the GK and HK enzymatic activity of WT cells, as well as un-induced or 5-day induced RNAiGK cells are shown in panel C. The anti-GK immune serum reveals non-specific bands in addition to GK, that is indicated by an arrow. nd means not detected and error bars in panels A and C indicate the means ± SD of 3 biological replicates.