Differential abundance volcano plot of eukaryotic microorganisms between infection states.

For ANCOM procedure, the clr (centered log ratio) table of ASVs grouped by genera was used, which was transformed to adjust to values from 0 to 1. The W value represents the number of times in which the hypothesis that the abundance of the microbial groups was the same in both infection states was rejected. A positive X axis means that a genus is more abundant in the Fasciola-negative group and a negative value of the X axis means that a genus is more abundant in the Fasciola-positive group. Genera with reject null-hypothesis> 95% were considered significant and are abbreviated as following: Pseudozyma (PSE), Neocallimastix (NEO), Pilobolus (PIL), Apiospora (API), Cyllamyces (CYL), Leptosphaeria (LEP), Buxtonella (BUX), Cystobasidiomycetes_X (CYS), Rhodosporidium (RHO), Hyphozyma (HYP), Talaromyces (TAL), Thelebolus (THE) and Filobasidium (FIL). (PDF)