This work focus on the generation and application of high-density protein arrays to study autoimmune diseases. For the generation of a cDNA-expression library from murine T-helper cells type 1 (Th1) the cDNA was directly cloned in the E. coli expression vector pQE30NASTattB. As T cells are implicated in immunological reactions, this TH1 expression library will be a source of recombinant proteins enabling the monitoring of the antibody repertoire in various autoimmune diseases. 65.000 clones were picked, and 200 of them were sequenced. The DNA sequence analysis shows T cell specific genes such as genes coding for IFN-γ and T cell receptor. The diversity of the T cell library is about 70-80%. The expression analysis identifies 12.100 expressi...