J Gene Med

In order to construct high-density, high content arrays, we have used cDNA libraries as a source of thousands of proteins and have developed a successful system for expression and purification of thousands of proteins in parallel. We have used these proteins to generate high density protein arrays on glass slides and demonstrates their potential applications in the profiling of the antibody repertoire of auto-immune patients, and the determination of the specificity or crossreactivity of antibodies. The optimization of antibody arrays will also be described