Urban water was supplied to the Roman city of Forum Julii (Fréjus, southeastern France) for at least 200 years by a 39.4 km long aqueduct, operating in AD 50. Two perennial springs were successively collected, the Foux and the Siagnole, located at the outlet of Triassic and Jurassic karstic reservoirs, respectively. In this study, we performed high-resolution PIXE (Particle- Induced X-ray Emission) measurements of Ca, Si, Fe and Sr concentrations on selected cross-sections of laminated carbonate sampled along the sidewalls, before and after the connection of the two collection channels. Seasonal variations of water composition, suspended sediment load and discharge are recorded by alternating clear and dark layers, and can be traced by thei...