The variability and accessibility of NO3-N in the soil derives from the complex interaction of mineralization and ecological conditions. The results showed that in unfertilized two-year rotation conditions for mineralization had the most dominant effects on distribution and dynamics of NO3-N. However, in the fertilized rotation NO3-N dynamic and distribution was a result of mineral N application, plant assimilation and mineralization. In our agro-ecological conditions year to year NO3-N variations were extensive. However, without the addition of nitrogen potential NO3-N released from the mineralization is not sufficient for achievement of high yields. Moreover, fertilization must be based on Soil Fertility Control System.Varijabilnost prist...