Physiochemical Evaluation and Determination of Chemical Constituent in Rose Petal (Rosa centifolia)

Objectives: The purpose of the present study was to explore the Pharmacognostic parameters for the standardization of Rosa centifolia Petals.  Material and Methods: The flowers of Rosa centifolia were authenticated and shade dried. Rosa centifolia petals were collected then macromorphological, physiochemical assessment, and the micrometric study was carried out. The dried powder form of the Rosa centifolia Petals was then extracted with different solvent systems alcoholic, ethyl acetate, and petroleum ether, and their extractive values were calculated. Most of the phytochemicals were found in ethanol and ethyl acetate fractions. Thin Layer chromatography (TLC) of the ethanol, ethyl acetate, and petroleum ether extract was performed for important phytochemicals flavonoids and polyphenol. Flavonoids and phenolics showed their presence in all extracts with one spot in each extract for ethanol, ethyl acetate, and petroleum ether. Result: The Physicochemical exploration showed values for moisture content, moisture sorption capacity, ash values, and extractive values which are within the limits of World Health Organization standards for the crude drug from medicinal plants. Micromeritic analysis of petal powder reveals good flowability. Ethanolic extractive values were found to be higher when compared to extractive values of, ethyl acetate and petroleum ether. Preliminary Phytochemical examination for the sample indicated the presence of carbohydrates, glycosides, alkaloids, flavonoids, and amino acids. Rf value for flavonoid and phenolic on TLC were found to be for ethanol 0.78 and 0.77, for ethyl acetate 0.81 and 0.78, for petroleum ether 0.81 and 0.78 respectively. Conclusions: The current research would be useful to supplement the information regarding pharmacognostical characteristics, physicochemical evaluation, micrometric analysis, and phytochemical exploration in the Ayurvedic system of medicine for its identification and medicinal use.  Keywords: Rosa centifolia, Macromorphological description, Physicochemical evaluation, Phytochemical screening, TLC screening.&nbsp