© 2017 American Chemical Society. Rapeseed is considered to be a promising sources for hydroprocessed ester and fatty acid (HEFA) jet fuel production as a means to address energy security and climate change mitigation. However, concerns have been raised about its impact on water, as large-scale biofuel production may place pressure on fresh water supplies and water quality. Water footprint (WF) analysis, when combined with water-focused life cycle assessment (LCA), can be an effective system analysis tool for water sustainability. This study developed a life cycle water footprint analysis informed by inputs from multiple models for rapeseed HEFA jet fuel production in North Dakota and evaluated the environmental impacts on water utilization...