Quantification of extracellular UDP-galactose

The human P2Y14 receptor is potently activated by UDP-glucose (UDP-Glc), UDP-galactose (UDP-Gal), UDP-N-acetylglucosamine (UDP-GlcNAc), and UDP-glucuronic acid. Recently, cellular release of UDP-Glc and UDP-GlcNAc has been reported, but whether additional UDP-sugars are endogenous agonists for the P2Y14 receptor remains poorly defined. In the present study, we describe an assay for the quantification of UDP-Gal with sub-nanomolar sensitivity. This assay is based on the enzymatic conversion of UDP-Gal to UDP, using 1–4-β-galactosyltransferase. UDP is subsequently phosphorylated by nucleoside diphosphokinase in the presence of [γ32P]ATP and the formation of [γ32P]UTP is monitored by high performance liquid chromatography. The overall conversion of UDP-Gal to [γ32P]UTP was linear between 0.5 and 30 nM UDP-Gal. Extracellular UDP-Gal was detected on resting cultures of various cell types, and increased release of UDP-Gal was observed in 1321N1 human astrocytoma cells stimulated with the protease-activated receptor agonist thrombin. Occurrence of regulated release of UDP-Gal suggests that, in addition to its role in glycosylation reactions, UDP-Gal is an important extracellular signaling molecule