The majority of the electricity supplied in Tasmania, Australia, is produced by hydropower. Hydro Tasmania, the power generation utility, operates 29 hydropower stations incorporating 170 km of open channels. These open channels are susceptible to extensive biofilm growth dominated by the freshwater diatoms Gomphonema tarraleahae and Tabellaria flocculosa, which form a gelatinous biofilm several millimetres thick and cause reductions in flow capacity of up to 10%. This thesis presents results of a multidisciplinary study on the effects of freshwater biofilms on hydropower canal capacity and turbulent boundary layer structure. The extent to which the surface roughness affects the structure of the turbulent boundary layer was critical...