Indirect photometric detection in capillary electrophoresis using buffered electrolytes. Part 2: Practical rules.

In the first part of this two-part series, the guest authors explained the principles and approaches to indirect detection and stressed the importance of buffering for method ruggedness. This second part outlines the different ways electrolytes can be buffered while maintaining compatibility with indirect detection and gives rules and practical guidelines for method development. The authors also discuss some instrumental aspects of indirect photometric detection that affect the method performance