POLYMORPHISM OF PRLR-GENE IN BLACK SLAVONIAN SOWS: PRELIMINARY RESULTS

Crna slavonska svinja, nekada vrlo cijenjena i gotovo jedina pasmina u Slavoniji, danas postoji u relativno maloj populaciji. Skromnih prohtjeva u hranidbi i smještaju, namijenjena je uzgoju na otvorenom. Prasi 6-8 odojaka u leglu, i prema tome pripada u srednje plodne pasmine svinja. Odlikuje se visokom kvalitetom mesa, namjenjenom preradi u tradicijske suhomesnate proizvode. Zbog svojih kvaliteta predstavlja prioritet u proučavanju i očuvanju jedinstvenog genetskog potencijala. Cilj ovog preliminarnog istraživanja bio je izolirati DNA iz folikula dlake 15 krmača crne slavonske pasmine svinja, provesti genotipizaciju polimorfizma prolaktinskog receptora (PRLR-gena) te utvrditi eventualnu povezanost pojedinih genotipova sa reproduktivnim pokazateljima. Navedeno bi moglo pridonijeti unaprjeđenju selekcije plotkinja, koja se dosad zasnivala na fenotipskim pokazateljima. Nakon provedene izolacije DNA i umnažanja odsječaka PRLR, dobiveni PCR produkti podvrgnuti su djelovanju restrikcijskog enzima AluI, u svrhu utvrđivanja polimorfizma PRLR-gena. Dobivena je jednaka raspodjela AA (40%) i BB (40%) homozigota, dok su AB heterozigoti bili manje zastupljeni (20%). Najveći broj ukupno oprasenih kao i živooprasenih odojaka imale su krmače AB genotipa, dok je kod BB homozigota zabilježena veća varijabilnost u veličini legla. Unatoč utvrđenim razlikama u veličini legla pojedinog genotipa, one nisu bile statistički značajne.Population of Black Slavonian pig, once highly valued and almost the only breed in Slavonia, has significantly decreased over the years. With modest demands of nutrition and housing, they are intendend for the breeding in the open. This breed belongs in the middle fertile breed with 6-8 piglets per litter. It is characterized by high quality meat, meant for processing in traditional dried meat products. Research and preservation of this unique genetic potential are priority because of its quality. The aim of this preliminary study was to isolate DNA from hair follicle samples of 15 Black Slavonian sows, to genotype polymorphism of PRLR receptor and evaluate its possible connection with reproductive traits. This could contribute to improvement of selection which previously was based on phenotypic indicators. After DNA isolation and multiplication of PRLR sequence, obtained PCR products were subjected to restriction enzyme AluI in order to determine the polymorphism of PRLR-gene. The results have shown even distribution of AA (40%) and BB (40%) homozygotes, while AB heterozygote was less frequent (20%). The largest number of total born and live born piglets was in AB genotype, although the BB homozygotes observed greater variability in litter size. Despite the determined differences in litter size between genotypes they were not statistically significant