A/J mice bearing the H-2 allele IAk are highly susceptible to autoimmune myocarditis induced with cardiac myosin heavy chain (Myhc)-α 334–352, whereas B10.A mice carrying a similar allele IAk are relatively resistant, suggesting that the generation of Myhc-α-reactive T cell repertoires is influenced by genetic background. To enumerate the precursor frequencies of Myhc-α-specific CD4 T cells, we sought to create IAk tetramers for Myhc-α 334–352. Tetramers were created using approaches that involve covalent tethering of individual peptide sequences or exogenous loading of peptides into empty IAk molecules by peptide-exchange reaction. Using ribonuclease 43– 56 tetramers as controls, we demonstrated that by flow cytometry (FC), Myhc-α 334–352 ...
Background: Tetramers are useful tools to enumerate the frequencies of antigen-specific T cells. How...
Abstract Background Tetramers are useful tools to enumerate the frequencies of antigen-specific T ce...
This report demonstrates the use of major histocompatibility complex (MHC) class II dextramers for d...
A/J mice bearing the H-2 allele IAk are highly susceptible to autoimmune myocarditis induced with ca...
A/J mice bearing the H-2 allele IAk are highly susceptible to autoimmune myocarditis induced with ca...
A/J mice bearing the H-2 allele IAk are highly susceptible to autoimmune myocarditis induced with ca...
Background—Cardiac myosin heavy chain-α (Myhc), an intracellular protein expressed in the cardiomyoc...
Enteroviruses like coxsackievirus B3 (CVB3) are common suspects in myocarditis/dilated cardiomyopath...
Background: Tetramers are useful tools to enumerate the frequencies of antigen-specific T cells. How...
Background: Tetramers are useful tools to enumerate the frequencies of antigen-specific T cells. How...
Background: Tetramers are useful tools to enumerate the frequencies of antigen-specific T cells. How...
Background: Tetramers are useful tools to enumerate the frequencies of antigen-specific T cells. How...
Background—Cardiac myosin heavy chain-α (Myhc), an intracellular protein expressed in the cardiomyoc...
Enteroviruses like coxsackievirus B3 (CVB3) are common suspects in myocarditis/dilated cardiomyopath...
Background: Tetramers are useful tools to enumerate the frequencies of antigen-specific T cells. How...
Background: Tetramers are useful tools to enumerate the frequencies of antigen-specific T cells. How...
Abstract Background Tetramers are useful tools to enumerate the frequencies of antigen-specific T ce...
This report demonstrates the use of major histocompatibility complex (MHC) class II dextramers for d...
A/J mice bearing the H-2 allele IAk are highly susceptible to autoimmune myocarditis induced with ca...
A/J mice bearing the H-2 allele IAk are highly susceptible to autoimmune myocarditis induced with ca...
A/J mice bearing the H-2 allele IAk are highly susceptible to autoimmune myocarditis induced with ca...
Background—Cardiac myosin heavy chain-α (Myhc), an intracellular protein expressed in the cardiomyoc...
Enteroviruses like coxsackievirus B3 (CVB3) are common suspects in myocarditis/dilated cardiomyopath...
Background: Tetramers are useful tools to enumerate the frequencies of antigen-specific T cells. How...
Background: Tetramers are useful tools to enumerate the frequencies of antigen-specific T cells. How...
Background: Tetramers are useful tools to enumerate the frequencies of antigen-specific T cells. How...
Background: Tetramers are useful tools to enumerate the frequencies of antigen-specific T cells. How...
Background—Cardiac myosin heavy chain-α (Myhc), an intracellular protein expressed in the cardiomyoc...
Enteroviruses like coxsackievirus B3 (CVB3) are common suspects in myocarditis/dilated cardiomyopath...
Background: Tetramers are useful tools to enumerate the frequencies of antigen-specific T cells. How...
Background: Tetramers are useful tools to enumerate the frequencies of antigen-specific T cells. How...
Abstract Background Tetramers are useful tools to enumerate the frequencies of antigen-specific T ce...
This report demonstrates the use of major histocompatibility complex (MHC) class II dextramers for d...