Gill nets set at three depths at an open-water station in Lake Titicaca during August 1984 captured only two species of endemic killifishes (Orestias ispi and o. mulleri) and an exotic silverside (Basilichthyes bonariensis). These species segregated strongly by depth, with B. bonariensis and o. mulleri occurring only in the surface and bottom sets, respectively, and O. ispi being most abundant in the mid-water set. All three species were feeding on zooplankton, but o. mulleri also included benthic crustaceans in its diet. Copepods were strongly selected by large (\u3e200 mm SL) B. bonariensis, whereas smaller size classes ate mainly cladocerans. Copepods and cladocerans were about equally represented in the average diet of adult O. ispi, bu...