The imagery of the ‘Rainbow’ Portrait of Elizabeth I which hangs at Hatfield House in Hertfordshire has been the subject of numerous academic investigations. However, few have addressed in satisfactory depth the many emblematic references that appear in the painting. Additionally, little research has been done to interpret the painting through an understanding of the material properties of the objects and clothing which it depicts. Through an interdisciplinary investigation this dissertation draws on evidence from Cesare Ripa’s emblem collection, the Iconologia (first published in 1593), combined with an examination of the Bacton Altar Cloth embroidery, c. 1590 -1600, currently being conserved by Historic Royal Palaces, supported by analysi...