© 2019 Elsevier B.V. A wide pore (1000 Å) diphenyl stationary phase was evaluated for the analysis of monoclonal antibodies (mAbs), comparing a conventional mobile phase of acetonitrile-water containing overall 0.1% trifluoracetic acid (TFA) with a similar mobile phase incorporating in addition 5% butanol. Alternatively, TFA was replaced by ammonium formate (AF) buffer (pH 3.0) and by methane sulfonic acid. Addition of 5% butanol to the mobile phase reduces the minimum temperature at which suitable UV analysis of the mAbs can be obtained from about 70 °C with TFA alone to about 60 °C thus potentially improving column lifetime and reducing the possibility of sample degradation. AF buffers produce satisfactory UV sensitivity at 70 °C and have...
The effect of changing the buffer at constant low pH in the mobile phase is investigated with respec...
Various recent wide-pore reversed-phase stationary phases were studied for the analysis of intact mo...
For the first time, the characterization of intact 150-kDa monoclonal antibodies (MAbs) using a comm...
© 2019 Elsevier B.V. A wide pore (1000 Å) diphenyl stationary phase was evaluated for the analysis o...
Reversed phase liquid chromatography (RPLC) of therapeutic monoclonal antibodies (mAbs) is often per...
Monoclonal antibodies (mAbs) represent one of the fastest growing areas of new drug development.Howe...
The separation performance of a chromatographic system is often described in terms of column efficie...
The present study describes the possibilities offered by an innovative bioinert size exclusion chrom...
When analyzing large complex protein biopharmaceuticals, ion-pairing agents imparting low pH are wid...
The development of monoclonal antibodies (mAbs) has been one of the hottest areas in the pharmaceuti...
Conventional reversed phase materials such as fully porous and superficially-porous packings current...
Recent reversed-phase wide-pore stationary phases were evaluated for the separation of intact monocl...
The goal of this work is to understand the phenomenon behind the poor injection repeatability ofteno...
Modern size exclusion chromatography (SEC) can be defined by the use of relatively small columns (e.g...
tCation exchange chromatography (CEX) of therapeutic monoclonal antibodies is generally performedwit...
The effect of changing the buffer at constant low pH in the mobile phase is investigated with respec...
Various recent wide-pore reversed-phase stationary phases were studied for the analysis of intact mo...
For the first time, the characterization of intact 150-kDa monoclonal antibodies (MAbs) using a comm...
© 2019 Elsevier B.V. A wide pore (1000 Å) diphenyl stationary phase was evaluated for the analysis o...
Reversed phase liquid chromatography (RPLC) of therapeutic monoclonal antibodies (mAbs) is often per...
Monoclonal antibodies (mAbs) represent one of the fastest growing areas of new drug development.Howe...
The separation performance of a chromatographic system is often described in terms of column efficie...
The present study describes the possibilities offered by an innovative bioinert size exclusion chrom...
When analyzing large complex protein biopharmaceuticals, ion-pairing agents imparting low pH are wid...
The development of monoclonal antibodies (mAbs) has been one of the hottest areas in the pharmaceuti...
Conventional reversed phase materials such as fully porous and superficially-porous packings current...
Recent reversed-phase wide-pore stationary phases were evaluated for the separation of intact monocl...
The goal of this work is to understand the phenomenon behind the poor injection repeatability ofteno...
Modern size exclusion chromatography (SEC) can be defined by the use of relatively small columns (e.g...
tCation exchange chromatography (CEX) of therapeutic monoclonal antibodies is generally performedwit...
The effect of changing the buffer at constant low pH in the mobile phase is investigated with respec...
Various recent wide-pore reversed-phase stationary phases were studied for the analysis of intact mo...
For the first time, the characterization of intact 150-kDa monoclonal antibodies (MAbs) using a comm...