D-Lys6 Salmon Gonadotropin-Releasing Hormone Analogue-Domperidone Induced Ovulation in Clarias batrachus (L.)

A single transmuscular injection of D-Lys6 salmon gonadotropin-releasing hormone analogue (sGnRH-A) at a dose of 50 µg•kg-1 body weight (b. wt) in combination with domperidone at 5 mg•kg-1 b. wt. induced final maturation and ovulation in Clarias batrachus. The fish did not spawn spontaneously but released eggs on handstripping. Fertilization and hatching rates were 70-80% and 50-60%, respectively. sGnRH-A alone did not induce ovulation at a dose as high as 500 µg•kg-1 b. wt. Comparable results in terms of spawning, fertilization and hatching were obtained when pituitary extract was injected at a dose of 40 mg•kg-1 b. wt