Thesis deals with analysis of payment instruments and systems in Czech republic during online shopping and how czech residents perceive them. In the first part is defined e-commerce and jurisdiction of payment systems. Then e-wallets (Paypal, Skrill and Czech alternative PaySec) are described. After that thesis deals with payment buttons and closely related payment agregators(PayU, Agmo, GoPay). Subsequently aquiring of credit cards is delineated. Last section of theoretical part of thesis deals with new method - payments with mobile phones (Mobito, MasterCard Mobile. In second part of thesis - practical part, there is analysed (with the help of primary survey and other provide surveys) actual situation of relation of czech residents to ele...