Isolation and Characterization of Microsatellite loci for Alligator Gar (\u3ci\u3eAtractosteus spatula\u3c/i\u3e) and their Variability in Two Other Species (\u3ci\u3eLepisosteus oculatus\u3c/i\u3e and \u3ci\u3eL. osseus\u3c/i\u3e) of Lepisosteidae

We report on the isolation of 17 polymorphic microsatellite loci from alligator gar (Atractosteus spatula), a large-bodied species that has experienced population declines across much of its range. These loci possessed 2-19 alleles and observed heterozygosities of 0-0.974. All loci conformed to Hardy-Weinberg equilibrium expectations, and none exhibited linkage disequilibrium. Nine and eight of these loci were found to be polymorphic in the related species Lepisosteus oculatus and L. osseus, respectively. These microsatellite loci should prove useful in conservation efforts of A. spatula through the study of population structure and hatchery broodstock management