Identifikacija prouzrokovača propadanja kruške u Srbiji

The symptoms pf pear decline are present in Serbia since 1970s. Thirty years later it was proved that the causal agent of this decline is a phytoplasma. This parasite is widespread in Europe and the USA as well as the countries of former Yugoslavia like Bosna and Hercegovina and Croatia. The presence of the phytoplasmas was confirmed using electron microscopy and the molecular analysis if the pear samples from a number of localities in Serbia between 2004-2007. The phytoplasma was observed on the ultrathin sections of the main leaf vascular bundle from the diseased pear tree. The shape, structure and size of 350-480 x 600-700nm, are typical ones for the phytoplasmas. PCR analysis using the universal primer R16F2n/R16R2 as well as the specific primer fO1/rO1 also confirmed phytoplasmas as the causal agent of pear decline.Simptomi propadanja kruške su prisutni u Srbiji od sedamdesetih godina prošlog veka. Mnogo godina kasnije, dokazano je da je prouzrokovač ovog propadanja fitoplazma kruške (PD). Ova pojava vrlo je raširena u Evropi i u Severnoj Americi, prisutna je i u susednim zemljama bivše Jugoslavije, Bosni i Hercegovini i Hrvatskoj. Prisustvo ove fitoplazme utvrđivano je primenom elektronske mikroskopije i molekularnom analizom uzoraka poreklom iz više lokaliteta u Srbiji, u periodu od 2004-2007 godine. Na ultratankim presecima floemskog tkiva glavnih lisnih nerava obolelih stabala kruške, utvrđeno je prisustvo organizama, koji po obliku, veličini (350-480 x 600-700nm) i strukturi, odgovaraju fitoplazmama. Rezultati PCR analiza, sa univerzalnim R16F2n/R16R2 i specifičnim prajmerom fO1/rO1 ukazali su na prisustvo fitoplazme Pear decline