Heterologous expression, purification, crystallisation and preliminary Xray analysis of raucaffricine glucosidase a plant enzyme specifically involved in Rauvolvia alkaloid biosynthesis.
- Ruppert, M.
- Panjikar, S.
- Barleben, L.
- Stoeckigt, J.
Publication date
January 2006
DOI Publisher
International Union of Crystallography (IUCr)Abstract
Raucaffricine glucosidase (RG) is an enzyme that is specifically involved in the biosynthesis of indole alkaloids from the plant Rauvolfia serpentina. After heterologous expression in Escherichia coli cells, crystals of RG were obtained by the hanging-drop vapour-diffusion technique at 293 K with 0.3 M ammonium sulfate, 0.1 M sodium acetate pH 4.6 buffer and 11% PEG 4000 as precipitant. Crystals belong to space group I222 and diffract to 2.30 A, with unit-cell parameters a = 102.8, b = 127.3, c = 215.8 A
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