PREPARASI NANOPARTIKEL KURKUMIN MENGGUNAKAN KITOSAN RANTAI PENDEK DAN TRIPOLIFOSFAT DENGAN VARIASI KONSENTRASI BERBEDA SERTA UJI CELULAR UPTAKE PADA KULTUR SEL HeLa SECARA IN VITRO

Curcumin, a natural polyphenolic compound with an anticancer properties presents in the turmeric (Curcuma longa L.). The optimum potensial for cancer therapy is limited by its poor oral biovability. The objectives of this study were to improve bioavability of curcumin by preparing curcumin nanoparticles using chitosan and tripolyphosphat at various concentrations, to characterize, to evaluate the stability of curcumin nanoparticles in artificial gastric fluid (AGF) and artificial intestinal fluid (AIF), to identify the cytotoxicity effect of curcumin nanoparticles against HeLa cell lines and normal cell lines (Vero) and to investigate the cellular uptake of curcumin nanoparticles. In this study, curcumin nanoparticles prepared by ionic gelation method using chitosan and TPP at various concentrations. The curcumin nanoparticles were then characterized for their physicochemical characterizations include particle size, zeta potensial morphology and entrapment efficiency. Furthermore, curcumin-chitosan nanoparticles were evaluated for their stability in artificial gastric fluid (AGF) and artificial intestinal fluid (AIF) by quantitating the release of curcumin from the nanoparticles as a function of time, cellular uptake of curcumin nanoparticles investigated using fluorescence microscopy and cytotoxic effect against HeLa cell lines and normal cell lines (Vero) by calculating the level of LC50 which was based on the percentage of the cell death following the 24 hours incubation with the nanoparticles. It was demonstrated that curcumin nanoparticles were prepared by ionic gelation technique using low viscous chitosan and TPP at various concentrations. The mean particle size of curcumin nanoparticles were 113,9 to 341, 2 nm, zeta potensial were (-)2,57 to (+)27,55, entrapment efficiency of curcumin in nanoparticles were 73,63 to 85,71% with the observed shapes of nanoparticles was spherical. Curcumin nanoparticles were stable in artificial gastric fluid and artificial intestinal fluid. Curcumin nanoparticles were more cytotoxic to HeLa cell line and there was a statistically significant difference in potensial cytotoxicity in HeLa cell lines in comparison to vero cell lines, cellular uptake studies of curcumin nanoparticles showed that nanoparticles were able to intenalize the HeLa cell lines