FORMULASI NANOKURKUMIN MENGGUNAKAN POLIMER KITOSAN RANTAI PENDEK DAN UJI CELLULAR UPTAKE PADA KULTUR SEL T47D

Curcumin as used as anticancer therapy has its restrictiveness from the case of its solubility and so that its low bioavailability, hopefully can be solved by the development of nanoparticle system. Nanoparticle technology has been started to be developed as an alternative to improve drug delivery, especially for the less bio-available chemicals. This study was aimed for developing nanocurcumin formulation with low viscous chitosan as the matrix and then for studying its ability to be taken into the cells in vitro. Methods used in the formulation of nanocurcumin is by ionic gelation followed by freeze drying. Entrapment Efficiency was then assayed, and its stability was tested by incubating into artificial intestinal fluid (AIF). Furthermore, the toxicity was evaluated, and also its cellular uptake ability into T47D cell line. It was found that the Entrapment Efficiency in acetate buffer at pH 4 is higher than at pH 5, it also has a good stability in AIF. For the cellular uptake study through fluorescence microscope, it was found that the complex has an ability to penetrate cellular membrane into the cytosol. Next, the cytotoxicity study gave us the information that the nanocurcumin is non-toxic to normal cell line. For the characterization of the nanoparticles, size measured as the average at 269.8 nm, Zeta-potential as +18.63 mV, with spherical particle morphology. From the result of this study, it is concluded that nanocurcumin using low viscous chitosan polymer as the matrix has a great potention as the alternative for anticancer therapy