Web 2.0 is a term describing the second stage of the web devel-opment in which static websites and presentations have trans-formed and continue to transform into a dynamic environment filled with user interaction and interpersonal communication. The aim of the diploma thesis was to introduce the reader to the term Web 2.0, describe the possibilities of its use and point out examples of successful application in practice. The first part of the diploma thesis tries to explain the term Web 2.0, define its basic properties and specify its typical ap-plications. The second part characterizes new generations of Internet users, provides description of the individual uses of Web 2.0 tools and services and summarizes their advantages and disadvantag...