Identifikasi Leptin setelah Luka Irisan padaTikus yang Diberi Pakan Lemak Tinggi

Leptin is a hormone produced by adipocytes and plays an important role in wound healing. The objectives of this research were to identify leptin and total leukocyte after incision on rat fed high fat diet and zinc topicalapplication. Sixteen male Sprague Dawley rats at 3 months of age were used in this study. Rats were randomly allotted into 4 groups (A,B,C and D) of 4. Rats in group A and B were fed normal diet, while rats in group C and D were fed high fat diet. After 2 months of treatment, skin incision was done at the back side of the rat. Incision wound was then closed with single interrupted suture. The wound of the rats in group A and C was treated with vaseline, while in group B and D was treated with zinc 10%. Three days after surgery, blood was collected from each rat for leptin analysis and total leukocyte. Leptin level and total leukocyte were analyzed statistically using ANOVA for factorial experiment (2x2). The results showed that leptin level was significantly higher in rats fedhigh fat diet compared to that of the rats fed normal diet (P<0.05). There was no significant different on total leukocyte. Therefore, it was concluded that leptin level was affected by diet, while total leukocyte was notaffected neither by diet nor topical application. However, total leukocyte tends to be lowered in the rat with zinc topical application.Keywords: high fat diet, leptin, zinc, total leucocyte, Sprague Dawley ratsLeptin merupakan hormon yang disintesis oleh sel lemak dan berperan dalam proses kesembuhan luka. Tujuan penelitian ini adalah mengidentifikasi leptin dan leukosit setelah luka irisan pada tikus yang diberi pakan lemak tinggi dan aplikasi topikal zinc. Penelitian ini menggunakan 16 ekor tikus Sprague Dawley jantan berumur tiga bulan (150-200 g). Tikus dibagi secara acak menjadi empat kelompok (A, B, C, dan D), masingmasing terdiri dari empat ekor. Kelompok A dan B diberi pakan normal, sedangkan kelompok C dan D diberi pakan lemak tinggi. Setelah dua bulan perlakuan pakan, seluruh tikus dioperasi untuk membuat luka irisan pada kulit, kemudian luka ditutup kembali dengan jahitan. Pada kelompok A dan C, luka jahitan diberi aplikasi topikal vaselin (tanpa zinc), sedangkan pada kelompok B dan D, luka jahitan diberi aplikasi topikal zinc 10%. Tiga hari setelah operasi, tikus diambil darahnya untuk analisis kadar leptin dan leukosit total. Data leptin dan total leukosit dianalisa dengan ANOVA untuk faktorial eksperimen (2x2). Hasil penelitian menunjukkan kadar leptin secara signifikan lebih tinggi pada tikus yang diberi pakan lemak tinggi dibandingkan tikus yang diberipakan normal (P