Аналіз впливу статевих гормонів на біохімічні показники стану серця щурів: зв’язок з рівнем гідроген сульфіду в міокарді

The aim of the work was to study the influence of different saturation of male and female rats’ organism with sex hormones on the parameters of pro-antioxidant system in heart and to assess their connection with the level of hydrogen sulfide in myocardium.Methods of research: The experiments were realized on 60 laboratory rats of both sexes. The experimental modeling of sex hormones level in rats’ organism was carried out by castration of animals under calypso anesthesia. The alternative hormone therapy in castrated male rats was carried out by administration of testosterone propionate, in females – estradiol hemihydrates. In myocardium was determined H2S content, activity of NAPD-oxidase, superoxide dismutase, level of malondialdehyde and carbonyl groups of protein. The level of testosterone and estradiol was assessed in heparin plasma. Results of research: H2S content and superoxidase dismutase activity are reliably less in male myocardium, whereas the activity of NAPD-oxidase, process of lipids and proteins peroxidase is reliably higher than in females. Male castration is attended with reliable increase of H2S content, superoxide dismutase activity and decrease of NAPD activity, lipids and proteins peroxidation processes activity in myocardium, whereas female castration caused the opposite changes. The alternative therapy with sex hormones approximated the indices to the level of control group of animals.Conclusions: high content of estradiol and low level of testosterone in blood plasma is associated with the high content of hydrogen sulfide in myocardium that is attended with the low activity of process of lipids and proteins free radical oxidation. The received data testify to the fact that the system of hydrogen sulfide in myocardium is an important factor of gender dimorphism of heart status in ratsМетою роботи було дослідити вплив різної насиченості організму самців та самок щурів статевими гормонами на стан про-антиоксидантної системи та вміст гідроген сульфіду в міокарді. Виявилось, що кастрація самців супроводжується зростанням в міокарді вмісту H2S та зменшенням активності пероксидації ліпідів та протеїнів, тоді як гонадектомія самок викликала протилежні змін