The paper aims to point out the subsistence in Eneolithic Central Italian communities by Stable Isotope Analysis. This period marked a tipping point in the food strategies because it was characterized by economic changes and several technological improvements leading to enhance land exploitation and livestock breeding. Carbon and nitrogen stable isotope analysis has been used to analyze the food consumption of 54 people belonging to 5 Eneolithic communities scattered throughout Central Italy, where no data have yet been published. The estimation of the main protein intake has been achieved in order to quantify the differences among these communities. The results are consistent with a diet mainly based on terrestrial resources, with no ...