Mitochondrial Genome of Nycticeius Humeralis

The purpose of my research was to sequence the mitochondrial genome of the Evening Bat, Nycticeius humeralis, and to create a phylogenetic tree based off of this data. The mitochondrial DNA was extracted using the Abcam Mitochondrial DNA Isolation Kit and sequenced on an Ilumina Nextseq at the University of Nebraska Medical Center. It was then assembled using Geneious and annotated via DOGMA. Finally, a phylogenetic tree was created with the mitochondrial genomes of closely related bat species and an outgroup on MEGA. I was unable to find any fully sequenced mitochondrial genomes of bats in the same genus as the Evening Bat.The first attempts at creating the phylogenetic tree produced unexpected results, showing the Evening Bat closest to our chosen outgroup and set apart from the bats deemed to be closely related to it. Therefore, the only conclusion I was able to draw was that the mitochondrial genome of more bats in the same genus as the Evening Bat need to be sequenced in order to create an accurate phylogenetic tree