Effect of Doses and Formulations of Bacterial Lipase on Gastric Emptying in Canine Exocrine Pancreatic Insufficiency

Background/Aims: There are few reports about the changes of gastric emptying (GE) in canine exocrine pancreatic insufficiency (EPI). In this study, we would like to determine whether bacterial lipase (BL) containing lipolytic activity alters GE in a dose-dependent way, different formulations of BL have different dose responses, and GE is affected by fat absorption. Methods: Five dogs underwent ligation of pancreatic ducts and insertion of duodenal cannulas. Perfusion and 72-hr fecal balance studies were performed while a high fat meal (fat 43%) was given. Powder or microtablet shaped BL were given at doses ranging from 0 to 400×103 IU. GE of liquid and solid meal markers was evaluated by total delivery (% total amounts delivered to duodenum), AUC (area under curve), T10 and T50 (minutes for 10% or 50% of ingested markers to arrive in duodenum). Results: GE of liquid meal marker was delayed in a dose-dependent way, but showed no formulation dependency. GE of solid meal marker was also delayed in dose-dependent way. Fat absorption was associated with delay of GE of liquid meal marker but it is not associated with GE of solid meal marker (p=0.0013). Conclusions: In dogs with EPI which took a high fat diet, GE of liquid and solid meal is delayed in a dose-dependent way, but not in formulation-dependent way. In addition, delay of GE of liquid meal correlates with fat absorption.ope