Effect of Doses and Formulations of Bacterial Lipase on Intestinal Transit in Canine Exocrine Pancreatic Insufficiency

BACKGROUND/AIMS: We determined in canine exocrine pancreatic insufficiency (EPI) when fed a high fat meal if 1) lipolytic activity as bacterial lipase (BL) alters intestinal transit (IT) dose dependently, 2) different formulations of BL have different dose responses, 3) correction of steatorrhea is related to IT. METHODS: Five dogs underwent ligation of a high fat meal (fat 43%) was eaten. Powder or microtablet BL were given at doses ranging from 0 to 400x103 IU. IT of liquid and solid meal markers was evaluated by total delivery (% total amounts delivered to ileum), AUC (area under curve), T10 and T4 (minutes for 10% or 4% of ingested markers to travel from duodenum to ileum). RESULTS: IT of the solid meal marker was not related to doses or formulations of BL. However, slowing of IT of the liquid meal marker was dose, but not formulation dependent (total delivery, p=0.0004; AUC, p=0.0017; T10, p=0.0245). Greater fat absorption was associated with slower IT of the liquid but not the solid meal marker (r2=0.262, p=0.0213). CONCLUSIONS: In dogs with EPI fed a high fat diet, slower IT of liquids is dose- but not formulation-dependent and slowing of IT of liquids correlates with greater fat absorption.ope