This study examined the inhibition of dentin demineralization in initial caries with Er : YAG laser irradiation only and laser irradiation combined with fluoride application. Dentin blocks were prepared from the roots of bovine incisors and pretreated with one of the following five procedures : A) untreated, B) Er : YAG laser irradiation for 5min at 50mJ/pulse, C) APF gel application for 5min, D) Er : YAG laser followed by APF application for 5min,and E) APF treatment for 5min followed by Er : YAG laser irradiation. Subsequently the samples were demineralized in a 0.1M lactic acid gel (pH5.0) containing 6wt% CMC at 37℃ for 2 weeks. The mineral distributions in the dentin surfaces were assessed quantitatively by transversal microradiography ...