In vitro Antimicrobial and AntioxidantProperties of Ganoderma lucidum ExtractsGrown in Turkey

Aim: To determine antimicrobial and antioxidative effects of Ganoderma lucidum.Place and Duration of Study: Erciyes University, Faculty of Pharmacy, PharmaceuticalBiotechnology research laboratory,Kayseri, Turkey, between January to March, 2013.Methodology: Antimicrobial inhibitory effects were carried out on the extracts using discdiffusion method. Antioxidant activities of the ethanolic and methanolic extracts fromG.lucidum were evaluated by using 2,2-diphenyl-1-picrylhydrazyl [DPPH] radicalscavenging, metal chelating, total flavonoid and total antioxidant activity assays. Inaddition, the amounts of phenolic compound, β-carotene and lycopene components in theextracts were determined.Results: The antimicrobial effects of ethanolic and methanolic extracts of G. lucidumwere tested against one species of Gram positive bacteria, two species of Gram-negativebacteria and two yeast. The highest inhibitory activity was determined against Candidaglabrata RSKK 04019 [25±1 mm, inhibition zone diameter]. On the other hand, the lowestinhibitory activity was determined against Candida albicans ATCC 90028 and Bacillussubtilis RSKK 244 [10±1 and 10±0 mm, inhibition zone diameter]. DPPH radicalscavenging effect was detected in the methanol extract [IC50 = 3.82±0.04 μg/mL] washigher than the ethanol extracts [IC50 = 7.03±0.07 μg/mL]. Compared to referenceantioxidant, the methanol and ethanol extracts of G.lucidum provided a lower IC50 thanbutylated hydoxyanisole [BHA] [IC50 = 0.30±0.01 μg/mL]. Phenolic compounds were themajor antioxidant component found in the mushroom extracts.Conclusion: These results showed that G. lucidum may be used in pharmaceuticalapplications because of its effective antioxidant properties.