Ispitivanje uticaja magnezijuma, cinka i bakra na izlučivanje kadmijuma kod kunića

Cadmium (Cd) is today one of the most significant metal poisons, both in the area of professional as well as of eco toxicology. In the organism, cadmium has a harmful effect on the kidneys, liver, bones, testicles, etc., and, based on evidence in humans that it causes lung carcinoma, it has been placed in the first group of carcinogens. In spite of numerous data in literature on the harmful effects of cadmium, the interactions between cadmium and bioelements as a significant mechanism for cadmium toxicity have still not been sufficiently explained. Since the data so far point to a positive effect of supplementation with certain bioelements regarding toxicity and cadmium content in the organism, the objective of this work was to investigate the effect of increased simultaneous intake of magnesium, zinc and copper on urinary elimination of cadmium in rabbits exposed to cadmium. Rabbits were divided into two groups: Cd group - for a period of 28 days the animals received per os 10 mg Cd/kg b.m/day and Cd+(Mg+Zn+Cu) group - 10 mg Cd/kg b.m. + 40 mg Mg/kg b.m, 20 mg Zn/kg b.m. and 10 mg Cu/kg b.m/day. Daily urine was collected on days 0, 10, 15, 17, 19, 21, 23, 25, and 28 of the experiment. Following decomposition of urine samples with the help of concentrated HNO3 and HClO4 (4:1), the metal concentration was determined using the method of atomic absorption spectrophotometry. Supplementation with magnesium, zinc and copper did not result in significant changes in the elimination of cadmium through urine in animals poisoned with cadmium, in comparison with the animals that were administered only cadmium, while the concentration of all three applied bioelements in urine was significantly increased. It can be concluded that the simultaneous administration of increased doses of zinc, copper and magnesium does not have a positive effect on the elimination of cadmium in conditions when rabbits are poisoned with cadmium.Kadmijum (Cd) je danas jedan od najznačajnijih metalnih otrova, kako u oblasti profesionalne tako i ekotoksikologije. U organizmu, Cd štetno deluje na bubrege, jetru, kosti, testise itd., a na osnovu dokaza da na ljudima izaziva karcinom pluća svrstan je i u I grupu karcinogena. I pored brojnih literaturnih podataka o štetnim efektima Cd, interakcije kadmijuma i bioelemenata kao značajnog mehanizma toksičnosti kadmijuma, još uvek nisu dovoljno razjašnjene. S obzirom na to da dosadašnji podaci ukazuju na pozitivan efekat suplementacije pojedinim bioelementima na toksičnost i sadržaj kadmijuma u organizmu, cilj ovog rada je bio da se ispita uticaj povećanog istovremenog unosa magnezijuma, cinka i bakra na urinarnu eliminaciju kadmijuma kod kunića izloženih kadmijumu. Kunići su bili podeljeni u dve grupe: I Cd grupa - životinje su 28 dana primale per os 10 mg Cd/kg t.m/dan i II grupa - Cd+ (Mg+Zn+Cu): 10 mg Cd/kg t.m. + 40 mg Mg/kg t.m, 20 mg Zn/kg t.m. i 10 mg Cu/kg t.m/dan. Dnevni urin je sakupljan 0, 10, 15, 17, 19, 21, 23, 25. i 28. dana eksperimenta. Nakon razaranja uzoraka urina uz pomoć koncentrovanih HNO3 i HClO4 (4:1), koncentracija metala je određena metodom atomske apsorpcione spektrofotometrije. Suplementacija magnezijumom, cinkom i bakrom nije dovela do značajne promene u eliminaciji kadmijuma urinom kod životinja trovanih kadmijumom u poređenju sa životinjama koje su primale samo kadmijum, dok je koncentracija sva tri primenjena bioelementa u urinu bila značajno povećana. Može se zaključiti da istovremeno davanje povećanih doza cinka, bakra i magnezijuma ne ispoljava pozitivan efekat na eliminaciju kadmijuma u uslovima trovanja kunića kadmijumom