(S)-2-amino-6-nitrohexanoic acid binds to human arginase i through multiple nitro-metal coordination interactions in the binuclear manganese cluster

The binding affinity of (S)-2-amino-6-nitrohexanoic acid to human arginase I was studied using surface plasmon resonance (K(d) = 60 microM), and the X-ray crystal structure of the enzyme-inhibitor complex was determined at 1.6 A resolution to reveal multiple nitro-metal coordination interactions