High-definition spatial transcriptomics for in situ tissue profiling
- Vickovic, Sanja
- Eraslan, Gökcen
- Salmén, Fredrik
- Klughammer, Johanna
- Stenbeck, Linnea
- Schapiro, Denis
- Äijö, Tarmo
- Bonneau, Richard
- Bergenstråhle, Ludvig
- Navarro, José Fernandéz
- Gould, Joshua
- Griffin, Gabriel K.
- Borg, Åke
- Ronaghi, Mostafa
- Frisén, Jonas
- Lundeberg, Joakim
- Regev, Aviv
- Ståhl, Patrik L.
DOIAbstract
Spatial and molecular characteristics determine tissue function, yet high-resolution methods to capture both concurrently are lacking. Here, we developed high-definition spatial transcriptomics, which captures RNA from histological tissue sections on a dense, spatially barcoded bead array. Each experiment recovers several hundred thousand transcript-coupled spatial barcodes at 2-μm resolution, as demonstrated in mouse brain and primary breast cancer. This opens the way to high-resolution spatial analysis of cells and tissues
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