An improvement of the metaphase finder system for the biological dosimetry by chromosome aberration

For biological dosimetry by counting chromosomes aberrations, automation technique has been required to process large number of sample preparations at low dose radiation exposure, or at large number of people who wants the exposure test. The metaphase finder is an automated optical microscope system, which automatically scans and finds metaphase cells on the slide glass, and relocates metaphase cells to the center of the field of view of the microscope to observe chromosomes in high magnification. We have constructed a Metaphase Finder system by assembling commercially available components, such as microscope, motorized sample stage, personal computer and general purpose image analysis software package, instead of purchasing one dedicated system.The new points of recent system were camera\u27s view field became larger than before, and morphology-based improved image processing method was used. Our system has begun to practically use in several domestic laboratories.The purpose of this paper is to test the performance of this new Metaphase Finder system. The results were: The scanning speed was 22 min/cm2. The false positive rate was 2 %.The speed was slower than several commercial metaphase finders but enough for practical use.The 13TH International Congress of RadiationResearc