The project of another low-cost metaphase finder

The most popular and “gold standard” phenomenon in Biological dosimetryis the appearance of dicentric chromosomes in metaphase in white blood cells. The metaphase finder is a tool for biological dosimetry that finds metaphase cells on slide glasses. The author and a software company were using new special software that was faster than conventional systems. We used a Nikon Eclipse Ni-E microscope with motorized X-Y stage, 4x objective lens and 1920 x 1024 pixels color camera for hardware. The software uses mathematical morphology filters. The new system was compact and low-priced. And the remarkable point is, this system can be applicable not only human blood, but also non-human samples. The speed was 208 to 236 seconds per 5 x 20 mm area, while capturing 378 images, which achieved the aim of the project. The false positive ratio achieved below 5 % in some slides